Porcine Hepatic Stellate Cells

Catalog No. P-6242

Suggested Medium: Complete Hepatic Stellate Cell Medium/w Kit – 500 ml

Catalog No. M5569

Product Description

Porcine Primary Hepatic Stellate Cells from Cell Biologics are isolated from porcine liver tissue. Cells are grown in tissue culture flasks and incubated in Cell Biologics’ Cell Culture Medium for 2-5 days. Prior to shipping, cells at passage 0 are detached from flasks and cryopreserved in vials.  Each vial contains 0.5x10⁶ cells per ml and is delivered frozen.

Product Testing

Porcine Primary Hepatic Stellate Cells from Cell Biologics are tested for expression of markers using antibodies, Desmin or α-actin by Immunofluorescence Staining. Cells are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded on a multiwell culture plate ready for experiments under the cell culture conditions specified by Cell Biologics. Hepatic Stellate Cells are not recommend for expanding or long term cultures since these cells would differentiate to become fibroblat-like cells immediately after plating and they will not proliferate in culture.

Laboratory Applications

Porcine Primary Hepatic Stellate Cells can be used in assays of standard biochemical procedures performed with cell cultures include RT-PCR, Western blotting, immunoprecipitation, immunofluorescent flow cytometry, or generating cell derivatives for desired research applications.

Storage of Cell Biologics Products

Cell Biologics ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen until ready for experimental use. Live-cell shipment is also available on request. Primary cells can never be kept at -20 °C.

Authorized Uses of Cell Biologics Products

Porcine Primary Hepatic Stellate Cells from Cell Biologics are distributed for internal research purposes only. Our products are not authorized for human use, for in vitro diagnostic procedures, or for therapeutic procedures. Transfer or resale of any Cell Biologics’ cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics, without the company’s written consent. Cell Biologics’ Terms and Conditions must be accepted before submitting an order.

Disclaimer

Appropriate safety procedures should always be used with this material. Investigators should handle the Cells that they receive from Cell Biologics with caution and treat all Cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents. The entire text of discussing Biosafety in Microbiological and Biomedical Laboratories, 5th ed. is available online at

http://www.cdc.gov/biosafety/publications/bmbl5/index.htm.

Warranty and Liability

Cell Biologics’ guarantee applies only to your purchase of Cell Biologics’ cells with Cell Biologics’ Media and Coating Solution, for appropriate cell culture and cell testing following Cell Biologics’ online protocols within 35 days from the date of product delivery.

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