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InformationRabbit Primary Vein Smooth Muscle Cells
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Rabbit Primary Vein Smooth Muscle Cells
Catalog No. N-6086
Suggested Medium: Smooth Muscle Cell Medium /w Kit – 500 ML
Catalog No. M2268
Custom Order (1-3 weeks)
Product Description
Rabbit Primary Vein Smooth Muscle Cells from Cell Biologics are isolated from inferior vena cava tissue of New Zealand White Rabbit. Rabbit Primary Vein Smooth Muscle Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based coating solution for 2 min and incubated in Cell Biologics Culture Complete Growth Medium generally for 3-7 days. Prior to shipping, cells at passage 1 are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 0.5x106 cells per ml and is delivered frozen. The cells are characterized by immunofluorescent method with antibody to α-smooth muscle actin (Monoclonal Anti-Actin, a-Smooth Muscle, A2547, Sigma). Rabbit Primary Vein Smooth Muscle Cells are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded on a multiwell culture plate ready for experiments under the cell culture conditions specified by Cell Biologics (Cells may be expanded for 2 or 3 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics). Repeated freezing and thawing of cells is not recommended.
Laboratory Applications
Rabbit Primary Vein Smooth Muscle Cells can be used in assays of standard biochemical procedures performed with cell cultures include RT-PCR, Western blotting, immunoprecipitation, immunofluorescent flow cytometry, or generating cell derivatives for desired research applications.
Storage of Cell Biologics Products
Cell Biologics ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use. Live-cell shipment is also available on request.
Primary cells never can be kept at -20 °C.
Authorized Uses of Cell Biologics Products
Rabbit Primary Vein Smooth Muscle Cells from Cell Biologics are distributed for research purposes only. Our products are not authorized for human use, for in vitro diagnostic procedures, or for therapeutic procedures. Transfer or resale of any Cell Biologics’ cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the company’s written consent. Cell Biologics’ Terms and Conditions must be accepted before submitting an order.
Disclaimer
Investigators should handle the cells that they receive from Cell Biologics with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.
Warranty and Liability
Cell Biologics’ guarantee applies only to your purchase of Cell Biologics’ cells with Cell Biologics’ Media and Coating Solution for appropriate cell culture and cell testing following Cell Biologics’ online protocols within 35 days from the date of product delivery.
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 Fetal Bovine Serum 6912 $0.00 |
Trypsin/EDTA 0.25% Solution 6914 $0.00 |
 Cell Culture Freezing Medium - ready to use 6916 $0.00 |
 Penicillin/Streptomycin Solution (1:100) 6917 $0.00 |
| Antibiotic/Antimycotic Solution (1:100) 6920 $0.00 |
Gelatin-Based Coating Solution- ready to use 6950 $0.00 |
Collage-I, Rat Tail Solution - ready to use 6953 $0.00 |
 Canine Primary Vein Smooth Muscle Cells D-6086 $0.00 |
 Tissue Lysis Buffer (1X) L6811 $0.00 |
 Cell Lysis Buffer (1X) L6822 $0.00 |
 Complete Smooth Muscle Cell Medium /w Kit – 500 ML M2268 $0.00 |
Smooth Muscle Cell Medium (No Phenol Red) /w Kit – 500 ML M2268PF $0.00 |
| Serum-Free Smooth Muscle Cell Medium /w Kit – 500 ML M2268SF $0.00 |
 Smooth Muscle Cell Basal Medium – 500 ML M2268b $0.00 |
