BALB/c Mouse Hepatic Stellate Cells

Catalog No. BALB-5242

Suggested Medium: Complete Hepatic Stellate Cell Medium/w Kit – 500 ml

Catalog No. M5569

 Product Description

BALB/c Mouse Primary Hepatic Stellate Cells from Cell Biologics are isolated from liver tissues of pathogen-free laboratory adult mice. Cells are grown in tissue culture flasks and incubated in Cell Biologics’ Cell Culture Medium for 2-5 days. Prior to shipping, cells at passage 0 are detached from flasks and cryopreserved in vials. Each vial contains 0.2x10⁶ cells per ml and is delivered frozen.

Product Testing

BALB/c Mouse Primary Hepatic Stellate Cells from Cell Biologics are tested for expression of markers using antibodies, Desmin or α-actin by Immunofluorescence Staining. Cells are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded on a multiwell culture plate ready for experiments under the cell culture conditions specified by Cell Biologics. Hepatic Stellate Cells are not recommend for expanding or long term cultures since these cells would differentiate to become fibroblat-like cells immediately after plating and they will not proliferate in culture.

Laboratory Applications

BALB/c Mouse Primary Hepatic Stellate Cells can be used in assays of standard biochemical procedures performed with cell cultures include RT-PCR, Western blotting, immunoprecipitation, immunofluorescent flow cytometry, or generating cell derivatives for desired research applications.

Storage of Cell Biologics Products

Cell Biologics ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen until ready for experimental use. Live-cell shipment is also available on request. Primary cells can never be kept at -20 °C.

Authorized Uses of Cell Biologics Products

BALB/c Mouse Primary Hepatic Stellate Cells from Cell Biologics are distributed for internal research purposes only. Our products are not authorized for human use, for in vitro diagnostic procedures, or for therapeutic procedures. Transfer or resale of any Cell Biologics’ cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics, without the company’s written consent. Cell Biologics’Terms and Conditions must be accepted before submitting an order.

Disclaimer

Investigators should handle the cells that they receive from Cell Biologics with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.

Warranty and Liability

Cell Biologics’ guarantee applies only to your purchase of Cell Biologics’ cells with Cell Biologics’ Media and Coating Solution, for appropriate cell culture and cell testing following Cell Biologics’ online protocols within 35 days from the date of product delivery.

Primary Cell Culture Protocol

• All cell culture procedures must be conducted in a bio-safety cabinet.
• Any and all media, supplements, and reagents must be sterilized by filtration through a 0.2 µm filter.
• Use aseptic technique to prevent microbial contamination.
• Cryo-preserved cells must be stored in liquid nitrogen or seeded immediately upon arrival.

Medium

• Review the information provided on the Cell Biologics’ website about appropriate culture media (e.g. serum and other supplements). Use pre-warmed (37°C) cell culture medium of M5569 (30-50 ML) to recover cryo-preserved cells and when changing media or splitting cells.

Cell recovery from cryovial

• Quickly thaw cells in cryo-vial by incubating them in a 37°C water bath for <1 min until there is just a small bit of ice left in the vial.
• Promptly remove the vial and wipe it down with 70% ethanol.
• Transfer cells from the vial to a sterile centrifuge tube. Add 6-8 ml of pre-warmed Cell Biologics Cell Culture Medium.
• Flush the vial with an additional 0.5-1 ml of medium to ensure complete transfer of cells to the centrifuge tube.
• Centrifuge cells at 200 g for 5 minutes.
• Aspirate the supernatant and resuspend the cell pellet in 3 ml of Cell Biologics’ Cell Culture Growth medium.
• Add resuspended cells into a Tissue-culture treated plate (one well of 6-well plate).
• Place a plate in a humidified, 5%-CO₂ incubator at 37°C until experiments.
• Change fresh cell culture medium next day and every 24-48 hours.
• Cells should be checked daily under a microscopy to verify appropriate cell morphology.

 Note

• Please send us the cell images (>90% confluence) if you have any question or problem with cultured cells.

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