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InformationDiabetic Mouse Mammary Fibroblasts
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Diabetic Mouse Mammary Fibroblasts
Catalog No. MD-6071
Suggested Medium: Fibroblast Medium /w Kit –500 ml
Catalog No. M2267
Product Description
Diabetic Mouse Mammary Fibroblasts from Cell Biologics are isolated from the breast of Diabetic (db/db) mice (8 weeks). Diabetic Mouse Mammary Fibroblasts are grown in T75 tissue culture flasks pre-coated with gelatin-based coating solution for 2 min and incubated in Cell Biologics’ Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells at passage 1 are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 0.5x106 cells per ml.
Product Testing
Diabetic Mouse Mammary Fibroblasts are tested for expression of marker using the antibody of anti-FSP1/S100A4 (Millipore USA) by immunofluorescence staining. Cells are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells is not recommended.
Laboratory Applications
Diabetic Mouse Mammary Fibroblasts can be used for the assay of cell-cell interaction, adhesion, PCR, Western blot, immunoprecipitation, immunofluorescent flow cytometry, or generating cell derivatives for desired research applications.
Storage of Cell Biologics’ Products
Cell Biologics ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use. Live cell shipment is also available on request. Primary cells should never be kept at -20 °C.
Authorized Uses of Cell Biologics’ Products
Diabetic Mouse Mammary Fibroblasts from Cell Biologics are distributed for internal laboratory research purposes only. Our products are not authorized for human use, not for in vitro diagnostiinternal c procedures or for therapeutic procedures. Transfer or resale of any Cell Biologics’ cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the company’s written consent. Cell Biologics’ Terms and Conditions must be accepted before submitting an order.
Disclaimer
Appropriate safety procedures should always be used with this material. Investigators should handle the cells that they receive from Cell Biologics with caution and treat all primary cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents. The entire text of discussing Biosafety in Microbiological and Biomedical Laboratories, 5th ed. is available online at http://www.cdc.gov/biosafety/publications/bmbl5/index.htm.
Warranty and Liability
Cell Biologics’ guarantee applies only to your purchase of Cell Biologics’ Cells with Cell Biologics’ Media and Coating Solution for appropriate cell culture and cell testing following Cell Biologics’ online protocols within 35 days from the date of product delivery.
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 Fetal Bovine Serum 6912 $0.00 |
Trypsin/EDTA 0.25% Solution 6914 $0.00 |
 Cell Culture Freezing Medium - ready to use 6916 $0.00 |
 Penicillin/Streptomycin Solution (1:100) 6917 $0.00 |
| Antibiotic/Antimycotic Solution (1:100) 6920 $0.00 |
Gelatin-Based Coating Solution- ready to use 6950 $0.00 |
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 Cell Lysis Buffer (1X) L6822 $0.00 |
| Complete Fibroblast Medium /w Kit – 500 ML M2267 $0.00 |
Serum-Free Fibroblast Medium /w Kit – 500 ML M2267SF $0.00 |
 Fibroblast Basal Medium – 500 ML M2267b $0.00 |
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