Lactate Dehydrogenase (LDH) Assay

Cat. #: CB001

Size: 2000 reactions

Kit components:

• Substrate Mix, lyophilizate, store at -20°C protected from light
• Assay Buffer, 0.6mL, store at -20°C protected from light.

Prepare the 2X LDH assay buffer as follows: Dissolve 223 mg INT (2-p-iodophenyl-3-p-nitrophenyl-5-phenyl tetrazolium chloride), 57 mg PMS (N-methylphenazonium methyl sulfate) , 575 mg NAD (nicotinamide adenine dinucleotide), and 3.2 g lactic acid in 480 mL 200 mM Tris buffer solution, pH 8.0.

• 10X Lysis Buffer, 9% Triton X-100. Dissolve 9 mL Triton X-100 with 91 mL milli-Q water, 2.5mL, store at 4°C
• Stop Solution, 1 M acetic acid, 12mL, store at 4°C
• LDH Positive Control, 6µL, store at 4°C

Additional Materials Required

• Cultured cell line

• Tissue culture 96-well plate

• Flat-bottom, clear 96-well plate compatible with spectrophotometry

• 1% bovine serum albumin (BSA) in phosphate-buffered saline (PBS)

• Multichannel pipette

• Spectrophotometer (ELISA) plate reader capable of reading 490nm and 680nm absorbance.

Have you ever published using Lactate Dehydrogenase (LDH) Assay? Submit your publication and earn rewards points which can be used for merchandise & discounts. Please include the product used, your name, email, publication title, author(s), PUBMED ID, Journal and issue in your submission.