Newsletter   |   Register   |   Log In   |   Cart(0)   
Shopping Cart
0 items
 
Information
 

NEWS


Cell Biologics' products can be purchased through Fisher Scientic by searching Cell Biologics' product catalog number or key words...

  • Medium Promotion, Buy 3 Bottles, get the 4th Free on Feb. 2018.

    Promo Code: CB0218.

Meet Us

AACR April 14-18, 2018 - Chicago

Experimental Biology April 22-24, 2018 - San Diego

PROMOTIONS


  • Get Free Gelatin-Based Coating Solution per primary cell order,  which can be used for most primary cell culture from Cell Biologics through 2018.

NEW PRODUCTS


 

Annexin V-Cy3 Apoptosis Detection Kit

Annexin V-Cy3 Apoptosis Detection Kit
Click to enlarge
Price: $0.00
Cat. #: CB002

Available Options:
Sequence:
Purity:
Elisa Kit:
Qty: Buy Quote

Related Documents:

Annexin V-Cy3 Apoptosis Detection Kit

Cat. #: CB002

(100 reactions)

Genebank Access Number: AAB60648.1

Kit components:                                            

Annexin V-Cy3: 500 µl  

SYTOX Green Dye: 100 µl            

Binding Buffer: 50 ml     

Description

After initiating apoptosis, most cell types translocate the membrane phospholipid phosphatidylserine (PS) from the inner face of the plasma membrane to the cell surface. Once on the cell surface, PS can easily be detected by staining with a fluorescent conjugate (Cy3) of Annexin V, a member of the annexin family of intracellular proteins that binds to phosphatidylserine (PS) in a calcium-dependent manner.  The Annexin V-Cy3 Apoptosis Detection Kit provides annexin V-Cy3, SYTOX green dye, and binding buffer. The SYTOX green dye is impermeant to live cells and apoptotic cells, but stains necrotic cells with intense green fluorescence by binding to cellular nucleic acids. After staining a cell population with annexin V-Cy3 and SYTOX Green dye in the provided binding buffer, apoptotic cells show red fluorescence, dead cells show green fluorescence and live cells show little or no fluorescence. These populations can easily be distinguished by Fluorescence microscopy using FITC and rhodamine filters or by flow cytometry using the FL1 channel (Ex=488 nm/Em=530 nm) for SYTOX Green dye and FL2 channel for Annexin V-Cy3 (Ex=543 nm/Em=570 nm).

Applications: Flow Cytometry, Fluorescence Microscopy

Storage: Store between 2°C-8°C. Do not freeze.

Write Review
Your Name:


Your Review: Note: HTML is not translated!

Rating: Bad            Good

Enter the code in the box below:

There are no additional images for this product.

Have you ever published using Annexin V-Cy3 Apoptosis Detection Kit? Submit your publication and earn rewards points which can be used for merchandise & discounts. Please include the product used, your name, email, publication title, author(s), PUBMED ID, Journal and issue in your submission.

No Publications Yet.
Related Products
Cat. # Name