Newsletter   |   Register   |   Log In   |   Cart(0)   
Shopping Cart
0 items
 
Information
 

NEWS


Cell Biologics' products can be purchased through Fisher Scientic by searching Cell Biologics' product catalog number or key words...

  • Medium Promotion, Buy 3 Bottles, get the 4th Free on Feb. 2018.

    Promo Code: CB0218.

Meet Us

AACR April 14-18, 2018 - Chicago

Experimental Biology April 22-24, 2018 - San Diego

PROMOTIONS


  • Get Free Gelatin-Based Coating Solution per primary cell order,  which can be used for most primary cell culture from Cell Biologics through 2018.

NEW PRODUCTS


 

Cell Senescence Assay

Cell Senescence Assay
Click to enlarge
Price: $0.00
Cat. #: CB011

Available Options:
Sequence:
Purity:
ELISA Kit:
Qty: Buy Quote

Related Documents:

Cell Senescence Assay

Cat. # CB011

(50 Tests in 35mm plate)

Description

Normal mammalian cells divide for a limited number of population doublings and eventually enter an arrested state in which the cells remain alive, but do not proliferate in response to mitogens, and assume a characteristic enlarged, flattened morphology. This process is senescence and thought to be a tumor suppressive mechanism and underlying cause of aging. Senescence-associated β-galactosidase (SA- β-gal) is a widely used biochemical maker for assessing senescence in cultured cells. The Cell Senescence Assay provide an easy-to-use method to detect SA-β-gal by staining cells with 5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside (X-gal) at pH 6.0, a pH condition that suppress lysosomal β-galactosidase activity sufficiently to ensure that nonsenescent cells remain unstained.

Kit Components

100X Fixing Solution: One tube, 1.5 mL of 25% Glutaraldehyde

Staining Solution A: One tube, 1.5 mL of 500 mM Potassium Ferrocyanide

Staining Solution B: One tube, 1.5 mL of 500 mM Potassium Ferricyanide

Staining Solution C: Three tubes,1.5 ml of 1 M Citrate-Na2HPO4 Buffer, pH6.0, 50 mM MgCl2

Staining Solution D: Two tubes – 2.0 mL of 5 M NaCl in each tube

X-gal Solution: Two tubes – 1.5 mL of 40 mg/mL X-gal in DMF in each tube

Materials Not Supplied

1. PBS

2. 37ºC Incubator

3. Light microscope

4. Senescent cells or tissue samples

*   Store X-gal solution protected from light at -20ºC. Store all other components at 4ºC. Crystal deposition, which comes from unreacted X-gal, may be observed after incubation of cells with working staining solution. It can be minimized by pre-filtering the working staining solution with a 0.2 µm filter.

Write Review
Your Name:


Your Review: Note: HTML is not translated!

Rating: Bad            Good

Enter the code in the box below:

There are no additional images for this product.

Have you ever published using Cell Senescence Assay? Submit your publication and earn rewards points which can be used for merchandise & discounts. Please include the product used, your name, email, publication title, author(s), PUBMED ID, Journal and issue in your submission.

No Publications Yet.
Related Products
Cat. # Name