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Cell Biologics' products can be purchased through Fisher Scientic by searching Cell Biologics' product catalog number or key words...

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Harvard Medical School Feb. 1, 2018 - MA

Experimental Biology April 22-24, 2018 - San Diego


  • Get Free Gelatin-Based Coating Solution per primary cell order,  which can be used for most primary cell culture from Cell Biologics through 2018.



Human Primary Colonic Fibroblasts

Human Primary Colonic Fibroblasts
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Price: $0.00
Cat. #: H-6231

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Primary Cells:
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Related Documents:

Human Primary Colonic Fibroblasts

Catalog No.  H-6231

Suggested Medium: Fibroblast Medium /w Kit –500 ml

Catalog No.  M2267


Product Description

Human Primary Colonic Fibroblasts from Cell Biologics are isolated from normal human colon tissue. Human Primary Colonic Fibroblasts are grown in T75 tissue culture flasks pre-coated with gelatin-based coating solution for 2 min and incubated in Cell Biologics’ Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells at passage 1 are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 0.5x106 cells per ml.

Product Testing

Human Primary Colonic Fibroblasts from Cell Biologics are tested for negative expression of von Willebrand Factor Expression/Factor VIII, cytokeratin 18, and alpha smooth muscle actin. Human Primary Colonic Fibroblasts are negative for bacteria, yeast, fungi and mycoplasma. Cells can be expanded for 3-5 passages at a split ratio of 1:2 or 1:3 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells is not recommended.

Laboratory Applications

Human Primary Colonic Fibroblasts can be used for the assay of cell-cell interaction, adhesion, PCR, Western blot, immunoprecipitation, immunofluorescent flow cytometry, or generating cell derivatives for desired research applications.

Storage of Cell Biologics’ Products

Cell Biologics ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C.

Authorized Uses of Cell Biologics’ Products

Human Primary Colonic Fibroblasts from Cell Biologics are distributed for internal research purposes only. Our products are not authorized for human use, not for in vitro diagnostic procedures or for therapeutic procedures. Transfer or resale of any Cell Biologics’ cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the company’s written consent. Cell Biologics’ Terms and Conditions must be accepted before submitting an order.


Appropriate safety procedures should always be used with this material. Although Human Primary Colonic Fibroblasts are isolated from normal human tissue, investigators should handle the cells that they receive from Cell Biologics with caution and treat all primary cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents. The entire text of discussing Biosafety in Microbiological and Biomedical Laboratories, 5th ed. is available online at

Warranty and Liability

Cell Biologics’ guarantee applies only to your purchase of Cell Biologics’ cells with Cell Biologics’ Media and Coating Solution for appropriate cell culture and cell testing following Cell Biologics’ online protocols within 35 days from the date of product delivery.

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Have you ever published using Human Primary Colonic Fibroblasts? Submit your publication and earn rewards points which can be used for merchandise & discounts. Please include the product used, your name, email, publication title, author(s), PUBMED ID, Journal and issue in your submission.

Title Author(s) Journal Issue

Growth hormone is permissive for neoplastic colon growth. Chesnokova V1, Zonis S1, Zhou C1, Recouvreux MV1, Ben-Shlomo A1, Araki T1, Barrett R2, Workman M2, Wawrowsky K1, Ljubimov VA1, Uhart M1, Melmed S3. Proc Natl Acad Sci U S A. 2016 Jun 7;113(23):E3250-9. doi: 10.1073/pnas.1600561113. 2016 May 25

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