Newsletter   |   Register   |   Log In   |   Cart(0)   
Shopping Cart
0 items
 
Information
 

NEWS


Cell Biologics' products can be purchased through Fisher Scientic by searching Cell Biologics' product catalog number or key words...

Meet Us

Harvard Medical School Feb. 1, 2018 - MA

Experimental Biology April 22-24, 2018 - San Diego

PROMOTIONS


  • Get Free Gelatin-Based Coating Solution per primary cell order,  which can be used for most primary cell culture from Cell Biologics through 2018.

NEW PRODUCTS


 

Rat Cell Protein Lysates

Cell Biologics provides custom services to our customers upon request. Please E-mail us your request toservice@cellbiologics.com. We will contact you as soon as possible.

Product Applications

Rat Cell Lysate provided from Cell Biologics is freshly isolated and ready-to-use for proteomics research applications, including electrophoresis, western blot, immunoprecipitation, enzymatic activity analysis, protein-protein interaction analysis, and tissue specific expression identification.

Background

Rat Cell Lysate is prepared by homogenization in modified RIPA buffer containing 150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, and protease inhibitor cocktail (66 μl/10 ml PBS, Sigma). Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.

Rat Cell Lysate 100-200ug:        Pricing

Endothelial Cell Lysate/T25Flask:               $580

Epithelial Cell Lysate/T25Flask:                  $510

Other Cell Lysate/T25Flask:                        $460-580

Storage Condition: at -80°C. until use.

Shipping:  Dry ice.

Rat Cell Lysates from Cell Biologics are distributed for in vitro research purposes only. Our products are not authorized for human use, for in vitro diagnostic procedures, or for therapeutic procedures.  Cell Biologics’Terms and Conditions must be accepted before submitting an order.

Thanks You!