SHR Rat Endothelial Cells
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Cell Biologics
SHR Rat Primary Endothelial Cells | ||||
(Spontaneous Hypertension) | Frozen Vial | |||
Catalog No. | Product Name | 1x106 Cells | T25 Flask | T75 Flask |
SHR-6008 | SHR Rat Carotid Artery Endothelial Cells | $560 | $650 | $790 |
SHR-6009 | SHR Rat Vein Endothelial Cells | $560 | $650 | $790 |
SHR-6011 | SHR Rat Lung Microvascular Endothelial Cells | $560 | $650 | $790 |
SHR-6014G | SHR Rat Kidney Glomerular Endothelial Cells | $560 | $650 | $790 |
SHR-6017 | SHR Rat Liver Sinusoidal Endothelial Cells | $560 | $650 | $790 |
SHR-6020 | SHR Rat Mammary Microvascular Endothelial Cells | $560 | $650 | $790 |
SHR-6023 | SHR Rat Brain Microvascular Endothelial Cells | $560 | $650 | $790 |
SHR-6024 | SHR Rat Cardiac Microvascular Endothelial Cells | $560 | $650 | $790 |
SHR-6027 | SHR Rat Prostate Microvascular Endothelial Cells | $560 | $650 | $790 |
SHR-6052 | SHR Rat Aortic Endothelial Cells | $560 | $650 | $790 |
SHR-6056 | SHR Rat Placental Microvascular Endothelial Cells | $560 | $650 | $790 |
SHR-6057 | SHR Rat Spleen Endothelial Cells | $560 | $650 | $790 |
SHR-6058 | SHR Rat Thyroid Microvascular Endothelial Cells | $560 | $650 | $790 |
SHR-6059 | SHR Rat Pulmonary Artery Endothelial Cells | $560 | $650 | $790 |
SHR-6060 | SHR Rat Pulmonary Vein Endothelial Cells | $560 | $650 | $790 |
SHR-6064 | SHR Rat Dermal Microvascular Endothelial Cells | $560 | $650 | $790 |
SHR-6064L | SHR Rat Dermal Lymphatic Endothelial Cells | $560 | $650 | $790 |
SHR-6065 | SHR Rat Retinal Microvascular Endothelial Cells | $560 | $650 | $790 |
SHR-6090 | SHR Rat Ovarian Microvascular Endothelial Cells | $560 | $650 | $790 |
SHR-6092 | SHR Rat Lymphatic Endothelial Cells | $560 | $650 | $790 |
SHR-6093 | SHR Rat Coronary Artery Endothelial Cells | $560 | $650 | $790 |
SHR-6206 | SHR Rat Pancreatic Microvascular Endothelial Cells | $560 | $650 | $790 |
SHR-6208 | SHR Rat Thymus Endothelial Cells | $650 | $650 | $790 |
SHR-6214 | SHR Rat Bladder Microvascular Endothelial Cells | $560 | $650 | $790 |
SHR-6219 | SHR Rat Uterine Microvascular Endothelial Cells | $650 | $650 | $790 |
SHR-6221 | SHR Rat Bone Marrow-Derived Endothelial Cells | $560 | $650 | $790 |
SHR-6054 | SHR Rat Small Intestinal Microvascular Endothelial Cells | $560 | $650 | $790 |
SHR-6055 | SHR Rat Intestinal Mesenteric Vascular Endothelial Cells | $560 | $650 | $790 |
SHR-6203 | SHR Rat Colonic Microvascular Endothelial Cells | $560 | $650 | $790 |
SHR-6220 | SHR Rat Skeletal Muscle Microvascular Endothelial Cells | $560 | $650 | $790 |
M1266 | Complete Rat Endothelial Cell Medium 500ml /w Kit | $118 | ||
Wistar Kyoto Rat Primary Endothelial Cells | ||||
Control cells for SHR cells | Frozen Vial | |||
Catalog No. | Product Name | 1x106 Cells | T25 Flask | T75 Flask |
WKY-6008 | WKY Rat Carotid Artery Endothelial Cells | $560 | $650 | $790 |
WKY-6009 | WKY Rat Vein Endothelial Cells | $560 | $650 | $790 |
WKY-6011 | WKY Rat Lung Microvascular Endothelial Cells | $560 | $650 | $790 |
WKY-6014G | WKY Rat Kidney Glomerular Endothelial Cells | $560 | $650 | $790 |
WKY-6017 | WKY Rat Liver Sinusoidal Endothelial Cells | $560 | $650 | $790 |
WKY-6020 | WKY Rat Mammary Microvascular Endothelial Cells | $560 | $650 | $790 |
WKY-6023 | WKY Rat Brain Microvascular Endothelial Cells | $560 | $650 | $790 |
WKY-6024 | WKY Rat Cardiac Microvascular Endothelial Cells | $560 | $650 | $790 |
WKY-6027 | WKY Rat Prostate Microvascular Endothelial Cells | $560 | $650 | $790 |
WKY-6052 | WKY Rat Aortic Endothelial Cells | $560 | $650 | $790 |
WKY-6056 | WKY Rat Placental Microvascular Endothelial Cells | $560 | $650 | $790 |
WKY-6057 | WKY Rat Spleen Endothelial Cells | $560 | $650 | $790 |
WKY-6058 | WKY Rat Thyroid Microvascular Endothelial Cells | $560 | $650 | $790 |
WKY-6059 | WKY Rat Pulmonary Artery Endothelial Cells | $560 | $650 | $790 |
WKY-6060 | WKY Rat Pulmonary Vein Endothelial Cells | $560 | $650 | $790 |
WKY-6064 | WKY Rat Dermal Microvascular Endothelial Cells | $560 | $650 | $790 |
WKY-6064L | WKY Rat Dermal Lymphatic Endothelial Cells | $560 | $650 | $790 |
WKY-6065 | WKY Rat Retinal Microvascular Endothelial Cells | $560 | $650 | $790 |
WKY-6090 | WKY Rat Ovarian Microvascular Endothelial Cells | $560 | $650 | $790 |
WKY-6092 | WKY Rat Lymphatic Endothelial Cells | $560 | $650 | $790 |
WKY-6093 | WKY Rat Coronary Artery Endothelial Cells | $560 | $650 | $790 |
WKY-6206 | WKY Rat Pancreatic Microvascular Endothelial Cells | $560 | $650 | $790 |
WKY-6208 | WKY Rat Thymus Endothelial Cells | $650 | $650 | $790 |
WKY-6214 | WKY Rat Bladder Microvascular Endothelial Cells | $560 | $650 | $790 |
WKY-6219 | WKY Rat Uterine Microvascular Endothelial Cells | $650 | $650 | $790 |
WKY-6221 | WKY Rat Bone Marrow-Derived Endothelial Cells | $560 | $650 | $790 |
WKY-6054 | WKY Rat Small Intestinal Microvascular Endothelial Cells | $560 | $650 | $790 |
WKY-6055 | WKY Rat Intestinal Mesenteric Vascular Endothelial Cells | $560 | $650 | $790 |
WKY-6203 | WKY Rat Colonic Microvascular Endothelial Cells | $560 | $650 | $790 |
WKY-6220 | WKY Rat Skeletal Muscle Microvascular Endothelial Cells | $560 | $650 | $790 |
M1266 | Complete Rat Endothelial Cell Medium 500ml /w Kit | $118 |
Product Description
SHR Rat Endothelial Cells from Cell Biologics are isolated from tissues of male, 9 week old laboratory SHR rats. Cells are grown in T75 tissue culture flasks pre-coated with gelatin-based coating solution for 2 min and incubated in Cell Biologics’ Culture Complete Growth Medium for 3-7 days. Cultures are then expanded. Prior to shipping, cells at passage 3 are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 0.5x106 cells per ml and is delivered frozen. The method we use to isolate primary endothelial cells was developed based on a combination of established and our proprietary methods. These cells are pre-coated with PECAM-1 (CD31) antibody, following the application of magnetic beads pre-coated with secondary antibody.
Product Testing
SHR Rat Endothelial Cells are tested for expression of markers using antibody, PECAM-1 Antibody (M-20, sc-1506, Santa Cruz) or ZO-1 Rabbit Polyclonal Antibody (Catalog No. 617300, Life Technologies) by immunofluorescence staining. Cells are also tested for uptake of Dil-Ac-LDL (Catalog No. L-35353, Invitrogen), a functional marker for endothelial cells. Cells are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded for 3-5 passages at a split ratio of 1:2 or 1:3 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells is not recommended.
Laboratory Applications
SHR Rat Endothelial Cells can be used in assays of cell-cell adhesion, migration, vascular tube formation, transendothelial resistance (TER). Standard biochemical procedures performed with cell cultures include RT-PCR, western blotting, immunoprecipitation, immunofluorescent staining, immunofluorescent flow cytometry, or generating cell derivatives for desired research applications.
Storage of Cell Biologics Products
Cell Biologics ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C.
Authorized Uses of Cell Biologics’ Products
SHR Rat Endothelial Cells from Cell Biologics are distributed for internal research purposes only. Our products are not authorized for human use, for in vitro diagnostic procedures, or for therapeutic procedures. Transfer or resale of any Cell Biologics’ cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics, without the company’s written consent. Cell Biologics’ Terms and Conditions must be accepted before submitting an order.
Disclaimer
Investigators should handle the cells that they receive from Cell Biologics with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.
Warranty and Liability
Cell Biologics’ guarantee applies only to your purchase of Cell Biologics’ cells with Cell Biologics’ Media and Coating Solution, for appropriate cell culture and cell testing following Cell Biologics’ online protocols within 35 days from the date of product delivery.