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NEWS


Cell Biologics' products can be purchased through Fisher Scientic by searching Cell Biologics' product catalog number or key words...

Meet Us

Harvard Medical School Feb. 1, 2018 - MA

Experimental Biology April 22-24, 2018 - San Diego

PROMOTIONS


  • Get Free Gelatin-Based Coating Solution per primary cell order,  which can be used for most primary cell culture from Cell Biologics through 2018.

NEW PRODUCTS


 

Uptake of Dil-Ac-LDL by endothelial cells

Uptake of Dil-Ac-LDL by endothelial cells


  • Cells are seeded in a 24-well plate coated with gelatin-based coating solution (catalog # 6950, Cell Biologics) for 5-10 minutes to 70–90% confluence.
  • Wash cells two times with 1% BSA in 1X PBS with Calcium & Magnesium (wash buffer).
  • Incubate cells with cell culture medium containing 0.05% FBS for 2 hours in an incubator (370C).
  • Remove cell culture medium from the plate.
  • Incubate endothelial cells with Dil-Ac-LDL at the concentration of 10 ug/ml for 4 h at 37°C. Note, mix Dil-Ac-LDL (Catalog No. L-35353/Alexa 594, Life Technologies) with complete cell culture medium containing 2-10 % FBS.
  • Decant the medium and wash the cells 3 times with wash buffer, 3 min each wash.
  • Keep the cells with 0.5-1.0 ml medium in the dark to analyze under a fluorescence microscope ASAP between 5 min - 4 hours (or fix cells and keep cells at 4°C in the dark until the scheduled time for analysis before 12-24 hours).
  • For the best result, analyze the cells as soon as possible after staining procedure.


CellBiologics