Next Generation Sequencing Library generation Services

As sequencing output increases and experimental scales are growing, generating libraries for sequencing is often the rate-limiting step. Cell biologics provides a fully integrated service for construction of high quality next-generation sequencing libraries to facilitate obtaining better data quality and less sequencing errors. Our library preparation services include library QC, library quantification, et al. All our libraries are specifically designed and optimized for any Illumina platform, and are already indexed (have a barcode).
We are happy to discuss the options and protocols suitable for your specific research projects. We prepare standard sequencing libraries as well as many specialized libraries and can also help you with custom projects. At the moment we are offering:

ATAC-Seq libraries

RNA-seq libraries

ChIP-seq libraries

DNA-seq libraries

miRNA-seq libraries (microRNA)

10X-Genomics GemCode libraries

scRNA-seq libraries (single cell RNA sequencing)

Methyl-Seq libraries

$200-250 per sample. 

For details regarding these special services contact us at service@cellbiologics.com.

Endothelial-Specific Aadenoviral Overexpression System

Introduction

Adenoviruses are widely used and powerful research tools for investigating virological and cellular events.  There has been a long-lasting interest in using viral vectors, especially adenoviral vectors, to deliver therapeutic genes for the past two decades. Among all currently available viral vectors, adenovirus is the most efficient gene delivery system in a broad range of cell and tissue types. Multiple adenoviral vectors were developed for in vitro and in vivo gene delivery. 

Expression system of adenoviral system has several advantages in vivo cell expression, ex vivo tissues or organs, and in vivo animal studies for

1)     molecular tool to study gene expression in difficult-to-transduce cell types

(endothelial, neural and other types of primary cell cultures);

2)     the production of high levels of recombinant, potentially therapeutic proteins;

3)     in vivo vaccination, for example, chimpanzee adenovirus vector Ebola vaccine;

4)     gene therapy.

Cell Biologics have developed a rapid, efficient endothelial specific adenoviral expression system for target gene delivery system in vitro cell culture and in vivo animal studies. The shuttle vector was incorporated by a 2.5 kb mice endothelial specific promoter in front of the multiple cloning sites vector to ensure target genes expressed only in endothelial cells for studying target gene functions in endothelial biology in vivo.  The universal adenoviral expression system allows customers to study the functions of endothelial-related target genes.

System contents:

a.     Endothelial specific PacAd5E shuttle vector (Catalog number: CB168V, ~$1000).

Notes: We can provide customer requested-cloning service to construct your target

genes into the universal and endothelial cell-specific in vivo expression vector.  We can also provide the ready-to-use recombinant adenovirus for customer request. Cost of specific cloning service depends on the length, structure complex of target genes.

b.     PacAd5E-100 backbone vector (Catalog number: CB166V, ~$500)

c.     PacAd5E-GFP control vector. (Catalog number: CB169V, ~$300)

Protocol:

The detailed protocol will be provided to customers.

Cell Biologics provides custom service upon request. If you have any special needs for this service, please contact us: service@cellbiologics.com.  We will contact you promptly within1 day.

Thank you!

Cell Biologics

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Cell Biologics provides custom services to our customers upon request. On request, we will isolate cells of any description from specific organs or tissues (any animal or human tissues) designated by our customers. For example, we are well versed in isolating endothelial cells and epithelial cells from animals (wild-type and knock-out murine strains, rat strains) from any desired tissue. Customer may ship to Cell Biologics either the already excised tissues or live mice or rats, and we will isolate the desired cell type and return a vial to Customer containing the prepared cells ready to use. If you have any special needs in isolation of endothelial cells, epithelial cells, macrophages, bone marrow derived cells or other cell types from animal or human tissues, please contact us for this special service. Let us know what you need and we will accommodate you.  

If you would like to use of Custom Cell Isolation Service, for details regarding these special services, please contact us at service@cellbiologics.com.

Thank you for your interest in Cell Biologics’ Cell Isolation Service and we look forward to working with you in the future.

Cell Biologics