Endothelial-Specific Aadenoviral Overexpression System

 

Introduction

Adenoviruses are widely used and powerful research tools for investigating virological and cellular events.  There has been a long-lasting interest in using viral vectors, especially adenoviral vectors, to deliver therapeutic genes for the past two decades. Among all currently available viral vectors, adenovirus is the most efficient gene delivery system in a broad range of cell and tissue types. Multiple adenoviral vectors were developed for in vitro and in vivo gene delivery. 

 

Expression system of adenoviral system has several advantages in vivo cell expression, ex vivo tissues or organs, and in vivo animal studies for

 

1)     molecular tool to study gene expression in difficult-to-transduce cell types

(endothelial, neural and other types of primary cell cultures);

2)     the production of high levels of recombinant, potentially therapeutic proteins;

3)     in vivo vaccination, for example, chimpanzee adenovirus vector Ebola vaccine;

4)     gene therapy.

 

Cell Biologics have developed a rapid, efficient endothelial specific adenoviral expression system for target gene delivery system in vitro cell culture and in vivo animal studies. The shuttle vector was incorporated by a 2.5 kb mice endothelial specific promoter in front of the multiple cloning sites vector to ensure target genes expressed only in endothelial cells for studying target gene functions in endothelial biology in vivo.  The universal adenoviral expression system allows customers to study the functions of endothelial-related target genes.

 

System contents:

 

a.     Endothelial specific PacAd5E shuttle vector (Catalog number: CB168V, ~$1000).

Notes: We can provide customer requested-cloning service to construct your target

genes into the universal and endothelial cell-specific in vivo expression vector.  We can also provide the ready-to-use recombinant adenovirus for customer request. Cost of specific cloning service depends on the length, structure complex of target genes.

 

b.     PacAd5E-100 backbone vector (Catalog number: CB166V, ~$500)

c.     PacAd5E-GFP control vector. (Catalog number: CB169V, ~$300)

 

Protocol:

The detailed protocol will be provided to customers.

 

Cell Biologics provides custom service upon request. If you have any special needs for this service, please contact us: service@cellbiologics.com.  We will contact you promptly within1 day.

 

Thank you!

 

Cell Biologics

Support

Special Services from Cell Biologics: Endothelial Cells from Genetically Modified Mouse Models.

Custom Cell Isolation: Complete our Customer Order Form and Email us: service@cellbiologics.com or submit Custom Inquiry Form on our website http://www.cellbiologics.net. We will contact your promptly within1 day.
Cell Biologics provides custom services to our customers upon request. On request, we will isolate cells of any description from specific murine organs or tissues designated by our customers. For example, we are well versed in isolating endothelial cells from wild-type and knock-out murine strains from any desired tissue. Customer may ship to Cell Biologics either the mouse or the already excised murine tissue, and we will isolate the desired cell type and return a vial to Customer containing the prepared cells ready to use. If you have any special needs in isolation of endothelial cells, macrophages, bone marrow derived cells or other cell types from wild type or knockout or other genetically modified mice, please contact us for this special service. Let us know what you need and we will accommodate you. For details regarding these special services contact us at service@cellbiologics.com or submit Custom Inquiry Form on our website http://www.cellbiologics.net. We will contact you as soon as possible.

Laboratory technical support services:

1. Vascular endothelial tube formation:an in vitro matrigel angiogenesis assay.
2. Agiogenesis in vivo within a Matrigel plug in mice
3. Endothelial cell (human and mouse) transfection with siRNA and cDNA
4. Endothelial cell expressing GFP (high efficient electroporation transfection)
5. Endothelial Cell transendothelial electrical resistance (TER)
6. Cell treatment
7. Imaging
8. PCR/RT-PCR
9. ELISA
10. FACS
11. Immunoprecipitiation
12. Immunoblotting
13. Calcium measurement assay
14. ROS measurement assay
15. Animal in vivo studies, including perfused mouse lung preparation (liquid and protein permeability)
    And More.

If you would like to use of Custom Cell Isolation Service, please contact us at service@cellbiologics.com for details regarding these special services. We will contact you as soon as possible!

Cell Biologics