The method we use to isolate primary stem cell-derived endothelial cells was developed based on a combination of established and our proprietary methods. These cells are isolated with anti-PECAM-1 antibody-conjugated magnetic beads.

Question 2: What passage number would they be?
You will receive stem cell-derived endothelial cells from passages 2 or 3, depending on cell purity. These cells can be used immediately after isolation.

Question 3: How many passages can primary stem cell-derived endothelial cells be maintained?
Primary stem cell-derived endothelial cells can be expanded 3-8 passages by the split ratio of 1:2 under the cell culture conditions specified by Cell Biologics.

The special cell culture media (M1168) are needed for maintaining custom-made cells.

Question 4: How many cells should you expect to receive from one order?
There are 1 million cells provided with each order depending on cell types; 2-4 million cells can be made as per your request.

The cells are frozen at one million cells/ml per vial or per request you can receive proliferating cells in a T75 flask/3 million cells.

Cell Biologics ships frozen cells on dry ice to anywhere in the world. On receipt, frozen cells can be transferred to liquid nitrogen until needed experimental use. Live cell shipment is also available on request.

Question 5: How long does it take for a custom cell culture?
Delivery time is usually 2-3 weeks from the time we receive your stem cell samples.

Question 6: How do we test our cells?
We test primary stem cell-derived endothelial cells for expression of markers using antibodies, VE-cadherin or Von Willebrand factor (vWF) by fluorescence staining. Also, you will receive cell morphology micrographs gratis on request and cell fluorescent antibody image will be provided for a modest fee.

Question 7: How is cell purity estimated?
Primary stem cell-derived endothelial cells produced from CellBiologics display typical "cobblestone" morphology under light microscopy and show VE-cadherin (CD144) staining at cell-cell junction or Von Willebrand Factor staining in plasma (see our website). These cells also express VE-cadherin and CD31 as demonstrated by FACS analysis.

Question 8: What laboratory applications can these cells be used for?
Primary stem cell-derived endothelial cells can be used for a variety of purposes (e.g., assays of cell-cell adhesion, migration, vascular tube formation, angiogenesis assays and many other applications) Standard biochemical procedures can be performed using endothelial cell cultures include RT-PCR, Western blotting, immunoprecipitation, or immunofluorescent staining or flow cytometry, et al.

Primary stem cell-derived endothelial cells from Cell Biologics are distributed for research purposes only. Our products are not authorized for human use. Transfer or resale of any Cell Biologics' cells or products from the purchaser to other markets, organizations, or individuals is prohibited by Cell Biologics without the express written consent of the company. Cell Biologics' Terms and Conditions must be accepted before submitting an order.

Question 9: How much does isolation of stem cell-derived endothelial cells cost?

a. $1200 for 1-2 million cells/frozen vial per custom order of one cell type.

b. $1200 for one T25 flask.

c. $1300 for one T75 flask.

Discount pricing is offered when orders are for more than three types of cells derived from different organs or tissues.

Question 10: What types of samples do we accept for cell culture for custom orders? 

You can send a frozen vial. You also can send live cells in a T25 or T75 flask. We will provide you with the specific protocol for sending us cells.

Question 11: Will Cell Biologics provide the isolation methods?
We will provide you with a brief synopsis of our cell isolation method for the purpose of your publication. All Cell Biologics methods are proprietary.

Question 12: What kind of support is provided for long term projects?
For long term use of our custom orders, one of our representatives will work with you on any special requests your project may entail, along with providing special discount pricing, upon approval of written contract.

Question 13: How do we inquire more about a custom order?
You can either send an email to service@cellbiologics.com with the details of your request including the type of cells needed and quantity of cells and one of our representatives will contact you for further information.

For a detailed list of the cells available from Cell Biologics, please go to our website at http://www.cellbiologics.net

If you would like to use of Custom Cell Isolation Service, please contact us at  sales@cellbiologics.com.

Custom Primary Cell Isolation Service is available internationally.

Thank you for your interest in Cell Biologics’ Cell Isolation Service and we look forward to working with you in the near future.

CellBiologics